The Lone Star Conference is an NCAA Division II conference.
Teams[]
| Team | Location | Stadium |
| Angelo State Rams | San Angelo, Texas | San Angelo Stadium |
| Central Washington Wildcats* | Ellensburg, Washington | Tomlinson Stadium |
| Eastern New Mexico Greyhounds | Portales, New Mexico | Greyhound Stadium |
| Midwestern State Mustangs | Wichita Falls, Texas | Memorial Stadium |
| Simon Fraser Clan* | Burnaby, British Columbia | Terry Fox Field |
| Texas A&M-Kingsville Javelinas | Kingsville, Texas | Javelina Stadium |
| Texas-Permian Basin Falcons | Odessa, Texas | Ratliff Stadium |
| West Texas A&M Buffaloes | Canyon, Texas | Kimbrough Memorial Stadium |
| Western New Mexico Mustangs | Silver City, New Mexico | Altamirano Stadium |
| Western Oregon Wolves* | Monmouth, Oregon | McArthur Field |
* - Associate members from the Great Northwest Athletic Conference.
Former teams[]
- Abilene Christian Wildcats
- Cameron Aggies
- Central Oklahoma Bronchos
- East Central (OK) Tigers
- Harding Bisons
- Houston Cougars
- Howard Payne Yellow Jackets
- Incarnate Word Cardinals
- Lamar Cardinals
- McMurry War Hawks
- North Texas Mean Green
- Northeastern State RiverHawks
- Oklahoma Panhandle State Aggies
- Ouachita Baptist Tigers
- Sam Houston State Bearkats
- Southeastern Oklahoma State Savage Storm
- Southwestern Oklahoma State Bulldogs
- Stephen F. Austin Lumberjacks
- Sul Ross State Lobos
- Tarleton State Texans
- Texas A&M-Commerce Lions
- Texas State Bobcats
- Trinity (TX) Tigers